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KMID : 0613820100200081254
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Journal of Life Science
2010 Volume.20 No. 8 p.1254 ~ p.1260
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Structural Identification of Antibiotics from Pseudomonas sp. RRj 228, a Antifungal Activity of Collectotrichum acutatum Causing Anthracnose on Pepper
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Jeon Sang-Yoon
Kim Yong-Gyun
Lee Sang-Mong
Son Hong-Joo
Park Hyean-Cheal
Kim Sun-Tae
Park Ki-Do
Kang Ui-Gum
Kim Keun-Ki
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Abstract
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Microorganisms near the plant rhizosphere usually inhabit the surface or the inside of the plant roots and have a direct effect on plant growth by secreting plant growth promoters or antagonistic materials which protect the root zone system from various pathogens. This study was carried out to identify and isolate the antagonistic materials after isolation of microorganisms showing high antagonistic activities, in hopes of contributing to the development of sustainable agriculture and the preservation of agricultural environments. A number of antagonistic bacteria were isolated from paddy soil. Among isolates, RRj 228 showed plant growth promotion and antagonistic activity. RRj 228 was identified as Pseudomonas sp. according to the results of physiological properties and genetic methods. On the basis of the results of anti-fungal spectrum against several pathogens by RRj 228, the antagonistic effect of the isolate against Botrytis cinerea, Pythium ultimum, Phytopthola capsici, and Rhizoctonia solani, especially against red-pepper anthracnose caused by Colletotrichum acutatum, was remarkable. The experiment evaluating the biological control effect by RRj 228 revealed that the ED50 value by the RRj 228 culture against C. acutatum, R. solani and P. ultimum were 0.14 ㎎/ml, 0.16 ㎎/ml and 0.29 ㎎/ml, respectively. An antagonistic substance was isolated and purified by several chromatographies from the RRj 228 culture. The ¹H and 13C assignment of the antagonistic substance was achieved from two-dimensional ¹H-¹H COSY, HMQC, and HMBC. Finally, the antagonistic substance was identified as Phenazine-1-carboxylic acid (C13H?N₂O₂, M.W.=224).
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KEYWORD
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Rhizobacteria, anthracnose, antifungal substances, phenazine-1-carboxylic acid
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